Scientists Bake Bread Using 5,300-Year-Old Yeast From Ötzi Mummy

These yeasts accompanied Ötzi on his long journey through the ages
A researcher describes how ancient microbes remained alive and active inside the 5,300-year-old frozen mummy.

More than five millennia after an arrow ended his life on an Alpine glacier, Ötzi the Iceman continues to yield revelations — this time, not from his bones or stomach contents, but from living organisms that never stopped existing within him. Scientists in Bolzano have cultivated ancient yeast from his intestines, baked bread with it, and in doing so have forced a quiet but profound reckoning: what we call preservation may in fact be a form of continuation, and the boundary between the frozen past and the living present is far more porous than we imagined.

  • Yeast isolated from a 5,300-year-old frozen mummy not only survived millennia of sub-zero entombment but proved capable of leavening bread after just three months of laboratory cultivation.
  • The discovery unsettles a foundational assumption in archaeology — that frozen remains are inert time capsules — revealing Ötzi's body as a dynamic, metabolically active ecosystem.
  • Early baking attempts failed repeatedly before researchers coaxed the ancient fungi into producing what they called a genuinely excellent natural starter, with beer fermentation now under consideration.
  • The same yeast can break down phenol, a chemical used to treat Ötzi's body at discovery, opening an unexpected path toward environmental remediation applications.
  • Ötzi's gut bacteria tell a parallel story: a microbe nearly extinct in industrialized populations persists in his remains, mirroring findings from Bronze Age salt miners and pointing to how profoundly modern diets have reshaped the human microbiome.

In September 1991, hikers on a glacier at the Italy-Austria border found a body so well preserved it seemed recent. It was not — Ötzi the Iceman had been frozen there since the Bronze Age, killed by an arrow some 5,300 years ago. He now rests in a Bolzano museum at minus six degrees Celsius, and for decades researchers assumed his remains were essentially static: a biological artifact, locked and unchanging.

That assumption collapsed when a team led by Mohamed Sarhan at Eurac Research found something alive in Ötzi's intestines, on his skin, and in the meltwater that collected when the mummy briefly thawed — four distinct species of yeast, the kind adapted to Antarctic cold and high mountain environments. Genetic analysis showed their DNA damage patterns matched those of microbes that had colonized Ötzi shortly after his death, suggesting the fungi had traveled with him across all fifty-three centuries. As coauthor Frank Maixner put it, these yeasts had accompanied Ötzi on his long journey through the ages.

The obvious next step, as Sarhan acknowledged with some humor, was to bake bread. The team cultivated the ancient fungi in a laboratory refrigerator. Early attempts failed. After three months of experimentation, they produced what Sarhan called a really very good natural starter — bread leavened by yeast that predates written history. Beer fermentation is now being explored.

The yeast's potential reaches beyond the kitchen. Because Ötzi's body was treated with phenol at discovery, and because the ancient fungi proved capable of degrading that compound, scientists believe it could one day assist in cleaning phenol-contaminated environments — a practical application from a 5,300-year-old organism.

Other surprises emerged from Ötzi's gut. A bacterium nearly absent from industrialized human populations — but still found in parts of Africa and South America — lived in his intestines, and the same microbe appeared in 3,000-year-old waste from an Austrian salt mine. Both Ötzi and those Bronze Age miners ate far more fiber and whole grains than modern people do, suggesting our microbiomes have shifted as dramatically as our diets. The study, published in Microbiome in June 2026, leaves a larger question open: if Ötzi's ancient microbes remain viable and reproducible after millennia, what else do we need to reconsider about preservation, about death, and about the persistence of life itself?

In September 1991, hikers crossing a glacier on the border between Italy and Austria stumbled upon a body so perfectly preserved that it seemed to belong to someone who had died only recently. They were wrong by 5,300 years. The man they found, known as Ötzi or the Iceman, had been frozen in place since the Bronze Age, killed by an arrow to the back. His discovery transformed him into one of archaeology's most valuable specimens—a window into a world most of us can only imagine.

Otzi's body now rests in a museum in Bolzano, Italy, kept at the exact temperature at which it was found: minus 6 degrees Celsius. The cold has done its work almost perfectly. His cells, locked in ice, retained their moisture and structure in a way that normal decomposition would never allow. For decades, researchers have picked through his remains, learning what he ate, what diseases he carried, how he lived. But they assumed the body itself was essentially static—a biological time capsule, frozen and unchanging.

That assumption turned out to be wrong. A team of scientists led by Mohamed Sarhan at the Eurac Research institute in Bolzano discovered something unexpected in Ötzi's intestines, on his skin, and in the brownish water that accumulated when the mummy partially thawed: yeast. Not just any yeast, but four distinct species capable of surviving in temperatures far below freezing. These are the kinds of fungi that live in places like Antarctica and high mountain ranges. They should not have been alive. Yet they were.

Genetic analysis revealed that the yeast's DNA bore damage patterns nearly identical to the original microbes that colonized Ötzi's body shortly after his death. This suggested the fungi had been with him for all 5,300 years, traveling through the millennia in his frozen remains. "These yeasts accompanied Ötzi on his long journey through the ages," Frank Maixner, a coauthor of the study, observed in a statement. The research, published in the journal Microbiome in early June 2026, fundamentally challenged the idea that a frozen body is simply a preserved artifact. It is, instead, a living ecosystem.

The discovery raised an obvious question, one that Sarhan acknowledged with a touch of humor: if you have yeast, why not make bread? The team cultivated the ancient fungi in a laboratory refrigerator and began experimenting. The first attempts failed. But after three months of trial and error, they produced what Sarhan described as "really very good natural starter." They had baked bread using yeast that had not been alive since before written history existed. The team is now considering whether the same microbes might be used to ferment beer.

The yeast's usefulness may extend beyond the kitchen. When Ötzi's body was first discovered, it was treated with phenol, a chemical meant to prevent fungal growth. The ancient yeast proved capable of breaking down phenol. Scientists now believe it could potentially be deployed to help decompose this chemical in contaminated environments—a practical application emerging from a 5,300-year-old organism.

The yeast was not the only surprise hidden in Ötzi's body. His intestinal microbiota contained a bacterium that has nearly vanished from modern humans living in industrialized nations, yet persists in some populations in Africa and South America. The same bacterium was found in 3,000-year-old human waste preserved in a salt mine in Austria. Both Ötzi and those Bronze Age salt miners consumed far more fiber and whole grains than people do today. The implication is clear: our microbiomes have changed as dramatically as our diets.

The study concludes that Ötzi is not a frozen capsule of biology, locked in time. He is something far more complex and alive. Nikolay Oskolkov, a researcher at the Latvian Institute of Organic Synthesis who had previously discovered fungi in the mummy but did not participate in this study, noted the significance of finding that the Iceman's microbiota is not actually frozen at all. The yeast samples were collected in 2010 and 2019, meaning the microbes had remained viable and reproducible for thousands of years. What we thought we understood about preservation, about death, about time itself, may need revision.

These yeasts accompanied Ötzi on his long journey through the ages
— Frank Maixner, coauthor of the study
The Iceman is not a biologically frozen time capsule, but rather a complex ecosystem
— Study conclusion in Microbiome journal
The Hearth Conversation Another angle on the story
Inventor

Why does it matter that yeast survived in a frozen body for five thousand years? Isn't that just what cold does—preserve things?

Model

Cold preserves, yes, but it doesn't kill everything. What's striking is that these microbes didn't just survive passively. They remained metabolically active. They could be grown. They could ferment. That changes how we think about what a frozen body actually is.

Inventor

So Ötzi isn't a time capsule. He's more like a living archive.

Model

Exactly. We assumed that freezing meant stopping—that biology was suspended. But the ecosystem inside him kept going, just very slowly. The yeast traveled with him through five millennia.

Inventor

The bread-baking part feels almost like a stunt. Why did they actually do that?

Model

It wasn't really a stunt. It was proof of concept. If you can grow the yeast, you can use it. And once you can use it, you start asking what else it might do. They're already thinking about phenol degradation, about environmental cleanup.

Inventor

What does the bacteria they found tell us about how we've changed?

Model

That our guts are different now. Ötzi ate fiber and whole grains. His microbiota reflected that. We don't. The bacteria that thrived in his intestines has nearly disappeared from ours. It's a record of how much our food—and therefore our bodies—have shifted.

Inventor

Does this change how archaeologists will study other frozen remains?

Model

It should. If Ötzi's body is a dynamic ecosystem, not a static snapshot, then every frozen mummy is too. We've been treating them as if they stopped changing the moment they froze. Now we know better.

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